We provide expertise and advanced equipment for Proteomics, Metabolomics and Bioenergetics

What services do we offer?

Our lab has state-of-the art technologies for advanced analyses of proteins, metabolites and metabolic activities, and offers collaboration within these areas.

Our work-flows cover steps from sample preparation, data acquisition and data treatment.

The technologies include:


  • Nano-liquid chromatography (LC) coupled to high resolution Mass Spectrometry (nanoLC-MS/MS), for targeted or un-targeted identification and quantification of proteins.
  • Luminex for multiplexed XMAP based protein quantification of e.g. inflammatory markers.


  • Ultra-high performance LC coupled to high resolution Mass Spectrometry, for targeted or un-targeted identification and quantification.


  • Seahorse metabolic analyzer XFe96 for quantification of cellular glycolysis and mitochondrial respiration.

The services are open for collaboration projects with actors from:

  • Aarhus University
  • Other Universities
  • Companies


The price of the services will be determined based on project specific factors, like:

  • Number of person hours
  • Costs of consumables
  • Mass spectrometry equipment hours


The collaboration projects are planned together with Associate Professor Johan Palmfeldt and other relevant experts.

The planning normally starts with an initial meeting followed by written information, and then the practical collaboration.

Guidance and Support

Guidance and support will be provided by the Core Facility, who has established protocols for a wide range of analyses.

The collaborator should specify:

  • type and content of the sample to be analyzed
  • analytical aim of the project.

Our yearly 4 day PhD courses could be of interest to some collaborators:

Course: Proteomics and protein profiling
The aim is to give the students an overview of proteomics and the study of complex protein samples. The course will cover the theoretical basis and practical methodology of proteomics, as well of the sister technology metabolomics.

Course: Gene to Function
The course covers theoretical background, the experimental methods, and interpretation of results in studies investigating consequences of mutations and molecular disease mechanisms.

Read more about our courses


Core Facility for Proteomics, Metabolomics and Bioenergetics is located at:

Olof Palmes Alle 49 (ground floor)
8200 Aarhus N

Publications and acknowledgements

In case of publication of data, to which the Core Facility contributed substantially, co-authorships are warranted (Vancouver recommendations).  The core facility can aid in the process of manuscript writing and in performing validation experiments, if needed.

See the list of publications associated with MMF.

Below are three recently published articles highlighted:

1. Metabolomics and proteomics data were combined in this patient-oriented study:

“Differences in the urinary metabolome and proteome between wet and dry nights in children with monosymptomatic nocturnal enuresis and nocturnal polyuria”

Jørgensen, C. S., Kamperis, K., Knudsen, J. H., Kjeldsen, M., Christensen, J. H., Borch, L., Rittig, S. & Palmfeldt, J., Oct 2023, In: Pediatric nephrology (Berlin, Germany). 38, 10, p. 3347-3358 12 p.


2. A strong mass spectrometry method for quantification of kynurenine metabolites was established:

“Simultaneous measurement of kynurenine metabolites and explorative metabolomics using liquid chromatography-mass spectrometry: A novel accurate method applied to serum and plasma samples from a large healthy cohort”

Eggertsen, P. P., Hansen, J., Andersen, M. L., Nielsen, J. F., Olsen, R. K. J. & Palmfeldt, J., Apr 2023, In: Journal of Pharmaceutical and Biomedical Analysis. 227, 12 p., 115304.


3. An advanced Nature Communication article by Degn et al benefitted from proteomics data from MMF.

“Antigen presentation by B cells enables epitope spreading across an MHC barrier”

Fahlquist-Hagert, C., Wittenborn, T. R., Terczyńska-Dyla, E., Kastberg, K. S., Yang, E., Rallistan, A. N., Markett, Q. R., Winther, G., Fonager, S., Voss, L. F., Pedersen, M. K., van Campen, N., Ferapontov, A., Jensen, L., Huang, J., Nieland, J. D., van der Poel, C. E., Palmfeldt, J., Carroll, M. C., Utz, P. J., & 3 others, Dec 2023, In: Nature Communications. 14, 1, 21 p., 6941.


About us

The Core Facility is a part of Research Unit for Molecular Medicine (MMF), Department of Clinical Medicine, HEALTH, Aarhus University, which is a fully equipped lab in molecular medicine.

See examples of our mitochondrial research and initiatives in translational medical research at Research Unit for Molecular Medicine (MMF).

Johan Palmfeldt has since 2010 been responsible for the proteomics platform, which gradually has been improved and extended to include also metabolomics.

Assoc. Prof Peter Bross is expert in Seahorse metabolic analyses, which likewise have been developing in recent years.

Assoc Prof Rikke Olsen is an expert in mitochondrial biology and genetics.